Annals of Case Reports and Clinical Studies (ISSN: 2834-5673) | Volume 2, Issue 3 | Research Article | Open Access
Prince Ogochukwu Alaebo*
Department of Biochemistry, College of Natural Sciences, Michael Okpara University of Agriculture Umudike, PMB 7267, Umuahia, Abia State, Nigeria
*Correspondence to: Prince Ogochukwu Alaebo
Fulltext PDFThe effect of bioremediation on sterile agricultural soil polluted with crude petroleum was investigated with a view to developing a suitable technique for the rehabilitation of similar environments when polluted with crude oil. Axenic cultures of non-pathogenic hydrocarbon utilizing bacteria namely; Pseudomonas aeruginosa and Bacillus subtilis were inoculated into the polluted soil to enhance the biodegradation action. Soil samples from the polluted soil were analyzed on a weekly basis for physicochemical and microbiological characteristics over sixty-three days study period. Hydrocarbon utilizing bacteria (HUB) isolated from the polluted soil include; Bacillus spp, Micrococcus spp, Pseudomonas spp, Acinetobacter spp, Flavobacterium spp, Alkaligenes spp, while the fungi include; Penicillum spp, Aspergillus spp, and Fusarium spp. Total Pseudomonas recovered from the experimental soil samples ranged from an average value of 0.6 ± 0.10 x 10-7 cfug -1 at week zero reaching up to 0.90 x 10 ± 0.15 cfug -1 at the middle of the sampling period, and finally to 0.7 ± 0.2 x 10 -7 cfug -1 at week nine. There was a marked reduction in the residual oil concentration especially in week nine which recorded a high 6.10 ± 0.02g/kg (93.90%) reduction and 7.60 ± 0.01g/kg (92.40%) reduction in experimental samples A and B respectively, when compared to an initial 88.0 ± 0.05 (12.00 %) and 81.6g/kg (18.40%) reduction in both samples at the beginning of the experiment. At 5% level of significant, almost equal ability was exhibited by both organisms in removing the oil pollutants.
Bioremediation, Crude oil pollution, Microbial community, Agricultural soil
Onuoha UN, Alaebo PO, Obike CA, Nwachukwu I, Okakpu JC, Muoneke BS, et al. Impact of Bioremediation on the Microbial Community of Crude Petroleum Polluted Agricultural Soil. Ann Case Rep Clin Stud. 2023;2(3):1-17.