International Clinical and Medical Case Reports Journal (ISSN: 2832-5788) | Volume 2, Issue 13 | Research Article | Open Access
Alaebo Prince Ogochukwu*
Department of Biochemistry, College of Natural Sciences, Michael Okpara University of Agriculture, Umudike, P.M.B. 7267. Umuahia, Abia State, Nigeria
*Correspondence to: Alaebo Prince Ogochukwu
Fulltext PDFIn vitro antibacterial activities of crude extract of Neem (Azadirachta indica) and Turmeric (Curcuma long Linn) were investigated against some bacterial isolates comprising of Salmonella typhi, Escherichia coli and Shigella dysenterae using the agar well diffusion and broth dilution techniques. The methanolic crude extract of both plants exhibited significant inhibitory action against the isolates tested at an initial concentration of 200mg/ml. The zones of inhibition exhibited by the extract against the tested organisms ranged between 10 and 28mm. There was a corresponding decrease in the zone of inhibition of the growth of the pathogens with decrease in extract concentration as was observed in the minimum inhibitory concentrations assay. The methanol extract of the leaves of Tumeric (Curcumin longa)exhibited a pronounced activity on Salmonella typhi by its wide zone of inhibition (28.0mm) when tested in its crude form. However, upon dilution at a minimum inhibitory concentration of 5%, a zone of inhibition of 8.0mm was observed against the same organism. Similarly, the same extract at its crude form was active against E. coli with a zone of 10.0mm and an MIC value of 0.1%. The potentials for developing antimicrobials from plants as revealed from this study seems rewarding as it will lead to the development of a phytomedicine against enteric pathogens.
Neem, Tumeric, Plant, Antimicrobial, Resistance
Onuoha Udumma Nsofor, Alaebo Prince Ogochukwu, Nwankwo Ifeanyi, Okakpu, Joseph Chinemelu, Okolie Jennifer Chisom. Antimicrobial Effects of Tumeric and Neem Leaves on Clinical Isolates. Int Clinc Med Case Rep Jour. 2023;2(13):1-7.