International Clinical and Medical Case Reports Journal (ISSN: 2832-5788) | Volume 2, Issue 5 | Research Article | Open Access
Associate Professor , SIMS College of Pharmacy, Mangaldas nagar, Guntur, IndiaFulltext PDF
A reserve phase liquid chromatography (RP-HPLC ) method has been developed and subsequently validated for the determination of Atenolol and Indapamide in bulk and its pharmaceutical formulation. Separation was achieved with anphenomenex C18 coloumn, 250mm x 4.6mm (particles with 5µm).A mixture of methanol and HPLC water (50:50)as mobile phase at a flow rate of 1 ml/min and the column temperature was maintained at 25˚c. Dual wavelength detector was performed at 231 nm with a run time of 10 minutes. The method was rapid, simple and sensitive. The described method of Atenolol and Indapamide is linear in the range of 10-50µg/ml with correlation coefficient of 0.996 for Atenolol and 0.997 for Indapamide respectively. The method enables accurate, precise and rapid analysis of Atenolol and Indapamide. It can be conveniently adopted for routine quality control analysis of bulk and pharmaceutical formulation.
RP-HPLC , HPLC methanol ,HPLC water , phenomenex C18 column
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